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28. Calculate the velocity of an enzyme- catalyzed reaction A+BP+Q at [A]= 2x 10-M and - 6.710 M. Assume that A and B add randomly to the enzyme and (a) a = 1 (the binding of one substrate has no effect on the binding of the other), (b) a = 0.1 (the binding of one substrate decreases the
dissociation constant for the other by a factor of 10). Preliminary experiments established that KA = 2.2 x 10-M, K, = 1.9x10-M, and V 7.21 = 72.7 nmoles > literx min.
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