Calibration Solutions Calibration Solutions Of Naproxen In The Range 5 25 Pg Ml Were Prepared Sample Preparation 20 Tab 1 (326.47 KiB) Viewed 22 times
Calibration solutions Calibration solutions of naproxen in the range 5-25 pg/mL were prepared. Sample preparation 20 tablets weighing 12.4856 g were crushed to a fine powder. A portion of the powder (149.8 mg) was shaken with approximately 150 mL of acetic acid (0.05 M) for 5 min and then made up to volume in a 250 mL volumetric flask (stock solution). Approximately 50 mL of the stock solution was filtered and a 25 mL aliquot was diluted to 100 mL in a volumetric flask. 10 mL of the resulting solution was further diluted to 100 mL with acetic acid (0.05 M). Analysis The standards and sample solutions were analyzed by HPLC under the following conditions: Column: octadecylsilyl (ODS), 4.6 mmx150 mm, mobile phase: acetonitrile : 0.05 M acetic acid (85:15), • flow rate: 1 ml/min, UV detection at 243 nm. Results: A calibration curve of concentration versus peak area was constructed for the standard solutions and gave the straight-line equation: y = 3555.6x +85, r = 0.9999 • The area of the naproxen sample peak = 45220 Stated content = 500 mg
y... 1 mobile phase, acetonitrile : 0.05 M acetic acid (85915), • flow rate: 1 ml/min, • UV detection at 243 nm. Results: • A calibration curve of concentration versus peak area was constructed for the standard solutions and gave the straight-line equation: y = 3555.6x + 85, r = 0.9999 • The area of the naproxen sample peak = 45220 • Stated content = 500 mg Calculate: a) The concentration of naproxen in the diluted sample solution analyzed Answer = pg/mL (2 decimal places) b) The actual amount of naproxen (in mg) in the assay powder weighed out. Answer = mg (1 decimal place) c) The actual amount of naproxen (in mg) per tablet. Answer = mg (1 decimal place) d) The percentage of stated content of per tablet. Answer = (1 decimal place)
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