4.1 How many fragments will form if pET28a(+) is digested simultaneously with SacI and BclI.4.2. Write down the base pairs that result at the 5' and 3' ends of the shortest fragment1314.3. Restriction enzymes often recognize palindromic sequences. What is a palindromic sequence?
4.1 How many fragments will form if pET28a(+) is digested simultaneously with SacI and BclI.
4.2. Write down the base pairs that result at the 5' and 3' ends of the shortest fragment
131
4.3. Restriction enzymes often recognize palindromic sequences. What is a palindromic sequence?
at pET-28bc (+) and pET-28c(t) e as pIT-28a(t) (shown) with hg cxceptions: pET-28b( +) is a imaid; subtract 1 bp from each site nHI at 198. pET-28c(+) is a maid; subtract 2bp from each site nht I at 198 .
at pET-28bc (+) and pET-28c(t) e as pIT-28a(t) (shown) with hg cxceptions: pET-28b( +) is a imaid; subtract 1 bp from ea
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at pET-28bc (+) and pET-28c(t) e as pIT-28a(t) (shown) with hg cxceptions: pET-28b( +) is a imaid; subtract 1 bp from ea
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