Following the successful ligation of the PCR insert and plasmid, Marie wants to amplify this new recombinant DNA plasmid

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Following The Successful Ligation Of The Pcr Insert And Plasmid Marie Wants To Amplify This New Recombinant Dna Plasmid 1 (168.72 KiB) Viewed 31 times

Following the successful ligation of the PCR insert and plasmid, Marie wants to amplify this new recombinant DNA plasmid (pET11−X). She wants to make sure that her recombinant plasmid consists of gene X. Assume Marie used the restriction enzymes BamHI \& EcoRI as shown in the schematic above in the original cloning strategy to insert gene X into pET11 creating pET11−X. Indicate the results of each restriction diagnostic assuming that gene X was successfully cloned into pET11 by indicating the appropriately sized DNA fragments. EcoRI: BamHI + EcoRI: Notl: EcoRV + HindIII: 13.a. If the sequences of the X gene are as follow, what would be the primer design? ( 2 ) EcoRI Hindil 5 5....A AGCTT..3"