7. Complete this Master Mix table for 3 DNA samples, a positive control, negative control, and an extra reaction for pip
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7. Complete this Master Mix table for 3 DNA samples, a positive control, negative control, and an extra reaction for pip
7. Complete this Master Mix table for 3 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your calculations work. Attach extra sheet if necessary. (5 pts) Mast er Mix PCR buffer Water dNTP mix MgC F Primer R Primer Taq polymer ase DNA Total volume of the entire reaction. (μL) Conc. Of Stock soluti on 20x 100 mM 40 mM 10 μM 10 μM 5 U/μL Fin al Co nc. 1X 10 μM 2 mM 0.2 μ. 0.2 μM 0.05 U/ μL μL/Rxn (total per tube) 1 μL 50 μL Number of Reactio ns Total μL neede d for mast er mix
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