6. Suppose that you were in the lab and are given the following bacteria to Gram stain: Escherichio coli and Bacillus su

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6 Suppose That You Were In The Lab And Are Given The Following Bacteria To Gram Stain Escherichio Coli And Bacillus Su 1 (54.37 KiB) Viewed 40 times

6. Suppose that you were in the lab and are given the following bacteria to Gram stain: Escherichio coli and Bacillus subtilis. You try the Gram stain several times on these organisms and follow the directions implicitly, but each time you conclude that you are decolorizing for too long because both controls contain pink cells (one more than the other). a. Note that in BIOL\&260, we will generally use Escherichia coli and Staphylococcus epidermidis as our Gram-negative and Gram-positive controls, respectively. We always run both Gram nositive and Gram negative controls on thesame slide as an unknown bacterium to ensure that b. The Gram negative cells in this question are. (Escherichia coll or Bacillus subtilis? Always follow the conventions of writing out bacterial names broperly. including both the genus and the species, etc.-. Gram negative cells should be (what color?) at the end of the Gram stain. c. The Gram positive cells in this question are. (Escherichia coli or Becillus subtilis? Always follow the conventions of writing out bacterial names properly, including both the Benus and the species, etc.). Gram positive cells should be (what color?) at the end of the Gram stain. d. As we will see later this quarter, the Gram positive cells in this question (and all bacteria in this genus) are sometimes problematic because (Hint: read the caption to Figure 3.50 ). The solution to this problem in this genus of bacterium is to use a bacterial culture,