Q2. You are on Part IV and need to calculate the volume of the enriched chloroplast suspension (from Tube "E") that you
Posted: Tue Jul 12, 2022 1:18 pm
Q2. You are on Part IV and need to calculate the volume of theenriched chloroplast suspension (from Tube "E") that you need toadd to 5 mL of cold sucrose buffer to get a standardized suspensionof 0.1 mg chlorophyll/mL. You follow steps 1-8 in the proceduresand measure an absorbance of 0.44 in Tube "L". From the absorbancevalue, first calculate the concentration of chlorophyll in Tube "E"(Cx). Then use that value to calculate the volume of Tube "E" (Vx)you need to add to 5 mL of buffer to get the standardized 0.1 mgchlorophyll/mL. Enter your answer for Vx (in mL) and include onlytwo places after the decimal and no units (eg. 1.48).