A membrane has three main types of membrane proteins shown on the diagram below: Integral membrane proteins (A) are embe

[answerhappygod]

A membrane has three main types of membrane proteins shown onthe diagram below:
Integral membrane proteins (A) are embedded in the lipidbilayer
Peripheral membrane proteins (B) are non-covalently associatedwith integral membrane proteins.
Amphitropic membrane proteins (C) associate with the membranevia direct lipid-protein covalent interactions.
Your goal is to experimentally determine the type of membraneassociation of three proteins (P1, P2, and P3).
In each experiment, a membrane sample is subjected to thefollowing steps:
Experiment 1: The membrane fraction is treatedwith SDS, and the solubilized sample is analyzed by SDS-PAGE. SDSis a anionic detergents that solubilizes lipid membranes andproteins. Proteins are visualized by staining the gel withCoomassie blue.
Experiment 2: The membrane fraction is treatedwith a sodium carbonate solution and then subjected tocentrifugation to separate the supernatant (soluble fraction S)from the pellet (membrane fraction M). The high pH (pH 11) of thesodium carbonate solution promotes the dissociation of ionicinteractions between protein and membranes. Fractions S and M areanalyzed by SDS-PAGE. Proteins are visualized by staining the gelwith Coomassie blue.
Experiment 3: The membrane fraction is treatedwith phospholipase C and then subjected to centrifugation toseparate the supernatant (soluble fraction S) from the pellet(membrane fraction M). Phospholipase C is an enzyme that cleavesthe linkage between proteins and their lipid anchors in membrane.Fractions S and M are analyzed by SDS-PAGE. Proteins are visualizedby staining the gel with Coomassie blue. The data are shownbelow.
Questions
a) Based on data from experiment 1, what canyou say about the type of membrane association for proteins P1, P2,and P3? Explain your answer.
b) Based on data from experiments 1 and 2, whatconclusion can you draw about the type of membrane association forproteins P1, P2, and P3 (integral, peripheral, amphitropic).Explain your answer.
c) Based on data from the three experiments,what conclusion can you draw about the type of membrane associationfor proteins P1, P2, and P3 (integral, peripheral, amphitropic).Explain your answer

A Membrane Has Three Main Types Of Membrane Proteins Shown On The Diagram Below Integral Membrane Proteins A Are Embe 1 (55.7 KiB) Viewed 51 times

Experiment 1 P1 1 P2 - P3 1 P2 P3 Experiment 2 M S I I -P1 P1 P2 Experiment 3 M I S I P3
A в с