Bioprocess engineering The time temperature data of heating and cooling periods of a 40000 lit tank during sterilisation

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Bioprocess engineering The time temperature data of heating and cooling periods of a 40000 lit tank during sterilisation

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Bioprocess engineering
The time temperature data of heating and cooling periods of a40000 lit tank during sterilisation of medium is
Time min 0-15,15-40,40-50,50-60,>60Temperature 85,121,85,55,30
The medium contains vitamins whose activation energy fordestruction is 10kcal/gmol and the value of Arhenious constant is10^4min ^-1.assume vitamin destruction is of first order and itsinitial concentration is 50mg/l.r is 1.99 cal/gmol*k.the mediumcontains 2.5*10^3 spores /lit.the spores have an activation energy65kcal/gmol and Kd at 121degree Celsius is 1.02min ^-1
estimate the probability of successful sterilisation
what fraction of vitamin remains active?
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