Problem 8. True or False. 8 points. a. The eukaryotic chromosome has multiple points of origin as this replication begin

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Problem 8 True Or False 8 Points A The Eukaryotic Chromosome Has Multiple Points Of Origin As This Replication Begin 1 (71.83 KiB) Viewed 49 times

Problem 8. True or False. 8 points. a. The eukaryotic chromosome has multiple points of origin as this replication begins. The prokaryotic plasmid has one OriC. b. During replication, bubbles forms at multiple points on the eukaryotic chromosome, a replication fork is formed and replication occurs at each point of origin until the many bubbles merge. c. Eukaryotic replication is not bidirectional and new DNA is laid down on the lagging strand in a 3'-to-5' direction because the lagging strand is the parental strand that is 5'-to-3' once the two DNA strands separate. d. For the eukaryotic DNA replication initiation complex, the lagging strand requires DNA polymerase alpha, DNA polymerase delta and the primase associated with DNA polymerase alpha. The leading strand only requires DNA polymerase alpha and the primase associated with DNA polymerase alpha. e. 'Okazaki fragments' are those sections of nascent DNA that are produced from the lagging strand in a 3'-to-5' direction. f. The RNA primer is no longer necessary once the replication processes toward nascent DNA that is 20 or more nucleotides long. In Okazaki fragments, the primer is removed by flap endonuclease 1 (FEN1) and RNase H. g. The gap left between two Okazaki fragments is filled by DNA polymerase alpha and its primase. h. Both eukaryotic replication and prokaryotic replication produce Okazaki fragments.