GNMENTS e Restriction Digest/Ligation worksheet Preview 0 2. In this set of labs, you will be restriction digesting lamb

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Gnments E Restriction Digest Ligation Worksheet Preview 0 2 In This Set Of Labs You Will Be Restriction Digesting Lamb 1 (69.32 KiB) Viewed 29 times

GNMENTS e Restriction Digest/Ligation worksheet Preview 0 2. In this set of labs, you will be restriction digesting lambda phage DNA and inserting that DNA into pUC19, the plasmid vector. Although lambda phage DNA has many restriction sites, only the sites for EcoRI (Figure 1) are mapped below (distance between the EcoRI sites noted in red). Note that only the lambda phage restriction digest products that contain two "sticky EcoRI ends can ligate into the plasmid forming a complete circle. E 21226 4878 E 5643 E 7421 26104 31747 Figure 1. Restriction map of lambda phage DNA for EcoRI Page < E 39168 5804 E of 2 44972 48502 ZOOM You will be cutting your lambda phage DNA and plasmid vector with EcoRI and then ligating the products. Note that four recombinant plasmids can be produced. Each recombinant plasmid will have a puC19 vector and one of the four possible inserts produced from the lambda phage digest. Using that information, draw out all possible recombinant plasmids that could be produced by the digestion and subsequent ligation.