Gamma-glutamylhydrolase catalyzes the hydrolysis of the peptide bond formed between the side chain carboxylate of glutam

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Gamma Glutamylhydrolase Catalyzes The Hydrolysis Of The Peptide Bond Formed Between The Side Chain Carboxylate Of Glutam 1 (102.72 KiB) Viewed 19 times

Gamma-glutamylhydrolase catalyzes the hydrolysis of the peptide bond formed between the side chain carboxylate of glutamate and the alpha-amino group of cysteine in the tripeptide substrate shown below. Gamma-glutamylhydrolase has a threonine residue that serves as the catalytic nucleophile in the reaction and the enzyme exhibits burst kinetics. a) Sketch a plausible mechanism for gamma-glutamylhydrolase. Explain your reasoning. -0OC, OH HO $ LOH H₂N +H₂N Gly 473 Thr 380 SH CH, O ||| CH-CH2-CH2-C-NH-CH-C-NH-CH,—COO H₂C -OH Gly 472 NH₂ 14 Ho .CH3 Thr 398 b) Shown below is a known inhibitor of gamma-glutamylhydrolase. Predict the type of inhibition. Sketch a Michaelis-Menten plot as a function of increasing inhibitor concentration. Label all salient features and justify your reasoning.