Bio 1111 Lab 4 Instructions: Cell Membranes and Transport Mechanisms Please type your answers in a different color or hi

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Bio 1111 Lab 4 Instructions: Cell Membranes and TransportMechanisms
Please type your answers in adifferent color or highlight them to make it easier for yourinstructor to read them.
PRE-LAB QUESTIONS
1. Read theintroduction to Procedure 4.1 and answer these questions:
2. Read the introduction to Procedure 4.2and answer these questions:
3. In Procedure4.3, you will expose plant cells to salt water. Read theintroduction to Procedure 4.3 and examine Figures4.3 and 4.4 to answer thesequestions:
Write your predictionhere:
This procedure was done for you. Allof the bags were set up just like in Figure 4.1. The weight of eachbag was recorded every 15 minutes for 45 minutes.
It is your job to take the weights ofthe bags and calculate the percent weight change for each timeinterval. The instructions on how to make this calculation areabove Table 4.2 in the lab book. Fill in the percent change columnsin Table 4.2, pasted below.
Percent Change: Remember toalways subtract the initial weight from the weight at each timeinterval. Remember to divide by the initial weight. If you want tocheck your percent change calculations, Google “percent changecalculator” and plug in the values in the table.
Follow the equations shown inthe lab book!
NOTE: If the bags LOSE weight, thenthe percent change values will be negative.
Table 4.2: Effect of time and sucroseconcentration on the weights of dialysis bags.
0 Min
15 Min
30 Min
45 Min
Bag
Initial Wt
(g)
Wt
(g)
% Wt change
(+/-)
Wt
(g)
% Wt change
(+/-)
Wt
(g)
% Wt change
(+/-)
A
22.5
22.6
21.5
20.3
B
21.7
22.5
22.7
22.5
C
23.0
24.6
24.7
25.9
D
23.0
25.5
26.7
28.4
Refer to the data in Table4.2 and plot a graph of time intervals andpercent weight change for each of the dialysis bags.Place time intervals (minutes) on thex-axis and the percent weightchange on the y-axis. Usedifferent colors or symbols for each of the four lines on thegraph. Clearly label the lines A, B, C or D. If bagslose weight, you will graph these as negative values. In thatcase, the zero point of your y-axis will not be at the bottom ofthe graph.
You can make your graph by hand on the graph paper in the labbook. Then, take a photo of the graph and paste the photobelow.
PHOTO:
Answer these questions. Be sure you understand the meaning ofthe terms, isotonic, hypertonic,and hypotonic.
Questions
Follow steps a, b, and c to fill inTable 4.3 below
a. Do step#3 in the lab book. Predict the outcome of exposing cells toeach solution. Will they stay the same, or gain, or lose water?Record your predictions in Table4.3. below.
Table 4.3: The results of exposing animalblood cells to solutions with different saltconcentrations.
Test Tubes
Solutions
Predictions
Translucent/
Opaque
Tonicity
1
5 mL distilled water
(0% salt)
2
5 mL 0.9% salt
3
5 mL 5% salt
TEST TUBE 1
0% NaCl (Distilled water + blood)
TEST TUBE 2
0.9% NaCl with blood
TEST TUBE 3
5% NaCl with blood