Substrate [S], μM 3 5 KM = 10 Vmax = 30 90 1/[S], μM-¹ Michaelis-Menten plot 0.33 0.20 0.10 0.03 0.01 Initial Velocity V

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Substrate [S], μM 3 5 KM = 10 Vmax = 30 90 1/[S], μM-¹ Michaelis-Menten plot 0.33 0.20 0.10 0.03 0.01 Initial Velocity Vo, μmol/min 10.4 KM = 14.5 Vmax = 22.5 Using graph paper or graphing program, make a Michaelis-Menten plot and a Lineweaver- Burke plot of the above data. From both graphs, determine KM and Vmax. 33.8 40.5 Lineweaver-Burke plot 1/vo, min/μmol 9.6 x 10-² 6.9 x 10-² 4.4 x 10-² 3.0 x 10-² 2.5 x 10-²
Initial velocity (μM product/s) Vo 50 45 40 35 30 25 15 10 5 - Do you have the same answers for KM and Vmax? If not, how did they differ? Which type of graph was best for finding the values of Vmax and KM? Why? V [S] max Km + [S] Vmax High [E] Km = 50 μM Vmax Low [E] 0 0 50 100 150 200 250 300 350 [S] (μM) 1 Vo 1 -8° Km Km V. [S] max. 1 Vmax + Slope = 1 Vmax Low [E] V 1 [S] 1 Slope = max Km Vmax High [E] Km Vmax