vp test- negative
indole- negative
catalase- negative
phenol red- positive
citrate- negative
oxidase- negative
lysine decarboxylase- negative
ornithine decarboxylase- negative
urease- negative
methyl red- positive
H2S- negative
with genera
Citrobacter
Yersinia
Serratia
Salmonella
Providencia
Proteus
Klebsiella
Hafnia
Escherichia
Edwardsiella
Erwinia
Enterobacter
Group 4 Gram negative rods and cocci, Aerobic/microaerophilic Bordetella - Coccobacilli, Respiratory metabolism, Mammalian pathogen, catalase positive, Indole positive, VP negative, non-motile, Urease negative, Oxidase positive Brucella -Cocci to short rod, non-motile, Respiratory metabolism, catalase, and coagulase positive, no acid from carbohydrate metabolism, VP negative, Methyl Red negative, Lysine decarboxylase negative, H2S positive, Urease positive Moraxella - short, plump rods usually appear in pairs, usually catalase positive, no acid from carbohydrates, urease negative, oxidase positive, non-motile Neisseria - Cocci in pairs, oxidase and catalase positive, non-motile Pseudomonas - Rods, motile, aerobic respiratory metabolism, catalase positive, oxidase results can vary, nitrate reduction Xanthomonas - Rods, motile, strictly aerobic respiratory metabolism, no nitrate reduction, uses carbohydrates and organic acids, oxidase negative, catalase positive Group 5.1 Gram negative rods, facultative anaerobe, most are motile, oxidase negative, catalase positive or negative within group, most reduce nitrates Citrobacter -Methyl red positive, VP negative, Citrate positive, Lysine decarboxylase negative, Urease negative, Indole negative Edwardsiella VP negative, Citrate negative, Lysine and Ornithine decarboxylase positive Enterobacter -VP positive, Citrate positive, usually Lysine decarboxylase negative, Ornithine decarboxylase positive, H2S negative, DNase negative, Lipase negative, Erwinia -Lysine and Omithine decarboxylase negative, nitrate reduction varies, ferments carbohydrates with acid but no gas, VP positive Escherichia -Methyl Red positive, VP negative, citrate negative, H2S negative, Urease negative, Lipase negative
Hafnia -Indole negative, Citrate negative, VP positive, Methyl Red positive, Lysine and ornithine decarboxylase positive, H2S negative, Urease negative Klebsiella -Lysine decarboxylase positive, non-motile, Ornithine decarboxylase negative, H2S negative, VP positive, Proteus -Methyl Red positive, Lysine decarboxylase negative, Urease positive, H2S positive, VP positive Providencia Methyl red positive, Indole positive, VP negative, Citrate positive Lysine and Ornithine decarboxylase negative, H2S negative, Urease negative Salmonella -Methyl Red positive, Indole negative, VP negative, Citrate positive, Lysine and Omithine decarboxylase positive, H2S positive, Urease negative Serratia - Indole negative, Citrate positive, VP positive, Lysine and Ornithine decarboxylase positive, H2S negative, Urease negative Shigella -non-motile, Methyl red positive, VP negative, citrate negative, Lysine decarboxylase negative, Omithine decarboxylase negative, H2S negative, Urease negative Yersinia -Methyl red positive, VP negative, citrate negative, H2S negative, Indole negative, lysine decarboxylase positive, Ornithine decarboxylase negative Group 5.2 Gram negative rod, facultative anaerobe, straight or curved rods, oxidase positive, motile Aeromonas -Omithine decarboxylase negative, Urease negative, Phenylalanine negative Vibrio -ferments glucose with no gas production, oxidase positive, found in aquatic habitats, Omithine decarboxylase positive Group 5.3 Gram negative rods, facultative anaerobe, coccoid to straight rod, non- motile, oxidase positive, reduces nitrates Haemophilus
-minute cell, requires blood for growth, oxidase and catalase results vary among species, glucose ferment with acid Pasteurella - Glucose fermented with acid with no gas, Methyl red negative, VP negative, Lysine decarboxylase negative Group 17 Gram positive cocci Enterococcus -cocci in chains, no endospores, fermentative metabolism with acid but no gas, catalase negative, facultative anaerobe, Beta hemolytic, motile Leucopostos. -cocci in pairs or chains, non-motile, no spores, facultative anaerobe, catalase negative, glucose ferment with acid and gas, indole negative, nitrates not reduced Micrococcus -Cocci in irregular clusters, strict erobic, rarely motile, glucose will not ferment to acid, catalase and oxidase positive Staphylococcus -cocci in single, pairs or irregular clusters, facultative anaerobe, both respiratory and fermentative metabolism, catalase positive, usually oxidase negative, nitrate reduction Streptococcus - cocci in chains, non-motile, fermentative metabolism with acid but no gas, facultative anaerobe, catalase negative Group 18 Gram positive rods, endospores Bacillus -rods, motile, aerobic (for our project), catalase positive Clostridium -rods, motile, strict anaerobic, catalase negative Group 19 Gram positive rod, irregular shape cell, no endospores Lactobacillus - - rarely motile, facultative anaerobe, fermentative metabolism, glucose ferments to acid or acid + gas, nitrates not reduced, catalase negative, oxidase negative Listeria -motile, facultative anaerobe, glucose ferments to acid no gas, catalase positive, oxidase negative, VP positive, Methyl Red positive, no nitrate reduction Group 20 Gram positive rods, irregular shape cell, no endospore Arthrobacter - irregular rods often in V shape, rod to small cocci, strict aerobic, catalase positive, VP negative, glucose ferments no acid or gas
Bifidobacterium -rods of varied shape, non-motile, strict anaerobic, ferments carbohydrates with no gas production, catalase negative Corynebacterium -rods sometimes with club end, non-motile, facultative anaerobe, glucose ferments to acid with no gas, catalase positive, gelatin hydrolysis negative Propionibacterium - pleomorphic rod often club-shaped, non- motile, facultative anaerobe, glucose ferment to acid and small amount of gas, catalase positive, gelatin hydrolysis positive Group 22 Gram positive filamentous rods, aerobic Nocardia -hyphae that easily fragment into rods or cocci, Gram (+) to Gram variable, strict aerobic, catalase positive, urease positive, nitrate reduction positive, VP negative, motile pecies List (by group) Group 4 Bordetella bronchiseptica Bordetella pertussis Bordetella abortus Brucella abortus Moraxella lacunata Neisseria gonorrhoeae Neisseria meningitidis Pseudomonas aeruginosa Xanthomonas campestris Group 5.1 Citrobacter freundil Edwardsiella tarda Enterobacter aerogenes Enterobacter cloacae Erwinia amylovora Escherichia coli Hafnia alvei Klebsiella pneumoniae Proteus mirabilis Proteus vulgaris Providencia alcalifaciens. Providencia rettaedi Salmonella choleraesuis. Shigella dysenteriae I
Serratia marcescens Yersinia enterocolitica Group 5.2 Aeromonas bydrophila Vibrio cholerae Group 5.3 Haemophilus influenzae Haemophilus parahaemolyticus Pasteurella multocida. Group 17 Enterococcus faecalis Leuconostoc lactis Micrococcus luteus Staphylococcus aureus Staphylococcus epidermidis Streptococcus salivarius. Group 18 Bacillus subtilis Clostridium butyricum Group 19 Lactobacillus delbrueckil Listeria monocytogenes Group 20 Arthrobacter globiformis Bifidobacterium bifidum Corynebacterium renale Propionibacterium acnes Group 22 Nocardia asteroide I
non-motile group 5.17,5.3 cxidase test negative group 5.1 (includes catalase) facultative anaerobe groups 5.1. 5.2, 5.3 motility gram negative groups 4, 5.1, 5.2, 5.3 positive group 5.3 thioglycollate broth Gram stain motile group 5.2 gram positive groups 17, 18, 19, 20, 22 aerobic/microaerophilic group 4 Focus
vp test- negative indole- negative catalase- negative phenol red- positive citrate- negative oxidase- negative lysine de
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