6. The structure of Bisbenzimide (Hoechst 33342) an organic compound used as a fluorescent stain for DNA in molecular bi

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6 The Structure Of Bisbenzimide Hoechst 33342 An Organic Compound Used As A Fluorescent Stain For Dna In Molecular Bi 1 (124.27 KiB) Viewed 29 times

6. The structure of Bisbenzimide (Hoechst 33342) an organic compound used as a fluorescent stain for DNA in molecular biology applications is give below. (2 pts) What is the degree of unsaturation for this molecule? State what you did to get your answer. i. ii. H3C-N As a student who is about to complete Organic chemistry I laboratory course what would you say the molecular formular of Bisbenzimide is? Using the molecular formular you just provided, determine the hydrogen deficiency index of Bisbenzimide. Does the value correspond with the degree of unsaturation you determined earlier? ΝΗ NH OH 2. Base on your understanding of IR spectroscopy what are the things you must consider when analyzing an IR spectrum and how do they aid in the analysis? (2 pts) 3. State at least 2 main weaknesses of IR spectroscopy. (1 pt.) 4. Why from your understanding are OH peaks generally broad? (1 pt.) 5. How will you use IR spectroscopy to distinguish between butanoic acid and 2- butanone? (2 pts)