An enzyme utilises a copper ion in its active site. The enzyme relies on the redox cycling of copper (Cu2+ + e- → Cu+) f

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An enzyme utilises a copper ion in its active site. The enzyme relies on the redox cycling of copper (Cu2+ + e- → Cu+) for its biological function. The donor atoms for the copper ion are four nitrogens (from histidine amino acids) and the reduction potential is 125 mV. A mutant of the enzyme is developed that has two of the histidines converted to cysteine amino acids