Morphine and morphine-3-β-D-glucuronide were separated using two different 50 x 4.6 mm columns with 3 µm particle. Colum

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Morphine and morphine-3-β-D-glucuronide were separated using two different 50 x 4.6 mm columns with 3 µm particle. Column A was C18-silica run at 1.4 mL/min and column B was pure silica run at 2.0 mL/min.a. Separation using column A was achieved using a mixture of 10 mM ammonium acetate (pH 3.0) and acetonitrile (98:2, v/v). Morphine and morphine-3-β-D-glucuronide eluted at 1.5 and 2.8 min, respectively. Calculate the retention volume and explain the order of elution.
Morphine and morphine-3-β-D-glucuronide were separated using two different 50 x 4.6 mm columns with 3 µm particle. Column A was C18-silica run at 1.4 mL/min and column B was pure silica run at 2.0 mL/min.a. Separation using column A was achieved using a mixture of 10 mM ammonium acetate (pH 3.0) and acetonitrile (98:2, v/v). Morphine and morphine-3-β-D-glucuronide eluted at 1.5 and 2.8 min, respectively. Calculate the retention volume and explain the order of elution.
Morphine and morphine-3-β-D-glucuronide were separated using two different 50 x 4.6 mm columns with 3 µm particle. Column A was C18-silica run at 1.4 mL/min and column B was pure silica run at 2.0 mL/min.
a. Separation using column A was achieved using a mixture of 10 mM ammonium acetate (pH 3.0) and acetonitrile (98:2, v/v). Morphine and morphine-3-β-D-glucuronide eluted at 1.5 and 2.8 min, respectively. Calculate the retention volume and explain the order of elution.