Culture When Doing The Streak Plate Technique A Loop Is Used To Streak The Mixed Sample Back And Forth Over The Surfac 1 (48.96 KiB) Viewed 28 times
Culture When Doing The Streak Plate Technique A Loop Is Used To Streak The Mixed Sample Back And Forth Over The Surfac 2 (42.09 KiB) Viewed 28 times
Culture When Doing The Streak Plate Technique A Loop Is Used To Streak The Mixed Sample Back And Forth Over The Surfac 3 (31.18 KiB) Viewed 28 times
Culture When Doing The Streak Plate Technique A Loop Is Used To Streak The Mixed Sample Back And Forth Over The Surfac 4 (30.99 KiB) Viewed 28 times
culture. When doing the streak plate technique, a loop is used to streak the mixed sample back and forth over the surface of a solid culture medium in a petri dish. As the cells are streaked across the plate, the cell density The streak plate technique is an isolation technique, which means it is done to isolate bacteria from a mixed decreases until individual cells are deposited onto the plate, eventually forming a colony. You will be conducting the quadrant streak plate today. Procedure: Obtain a nutrient agar plate and label with your initials, date, and culture number. You may want to divide your plate into four quadrants and label them. Obtain the mixed culture test tube. Shake the culture tube to mix the bacteria. Sterilize and cool down the inoculating loop. • Open the mixed culture tube, flame the neck of the tube and dip the inoculating loop into the mixed culture to obtain the mixed culture on the inoculating loop. Lift one edge of the petri plate cover and streak the first sector by making streak in 1/3 of the plate without overlapping with each other. Refer to Fig 1. Think about the inoculating loop as a paint brush and softly make these streaks on the surface without breaking the surface of the age. . . . . . . Sterilize the inoculating loop and cool it down. •Te streak the and test tube of mixed culture. To streak the second sector, overlap few streaks from the first sector and few streaks without any overlap from the first sector. Refer to Fig 2. • Sterilize and cool down the inoculating loop. To streak the third sector, overlap few streaks from the second sector and few streaks without overlapping (Fig 3) Sterilize and cool down the inoculating loop. To streak the fourth and the last sector, overlap few streaks from the third sector and few streaks without overlapping (Fig 4). • Sterilize the inoculating loop before setting it down. Cover the petriplate and incubate it upside down for 24 hours. Then observe your results. Stere the loop Streak Plate Results: Mivel Culture sectors, it is important to remember to not dip the inoculating loop into the. Fig 3 3rd Sterilize the loop 2nd Figz Fig4 Stke the koop Sterize the loop
3" Sector 4 Sector Sample 1 Sample 3 2nd Sector 1 Sector 2nd Sector 2nd Sector 1 Sector 1s Sector Sample 2 3rd Sector 4th Sector Sample 4 2nd Sector 4th Sector 1st Sector 3d Sector
BIO2073: Microbiology Lab Lab Report: Streak Plate 1. What is the purpose of the streak plate technique? (1 pt) Date: 2. After receiving a mixed culture you performed the streak plate technique and incubated the plate overnight at 37°C. Based on the results below, how many different species are present in your original culture? Explain your reasoning. (2 pts) 3. In which quadrant should you get isolated colonies? What are some two reasons you may not get isolated colonies in that sector? (3 pts)
4. You performed the streak plate technique and got the following result. Critique your results and explain how you can improve your technique. (2 pts) ASM MicrobeLibrary Ⓒ Liao 5. Your classmate performed the streak plate technique and got the following result. Explain to your friend what you think happened and what could be done to improve their technique. (2 pts) ASM MicrobeLibrary.org Katz 1
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