The next two questions could be selected for 5 point essay questions. You will be asked to draw the operon structure. Th
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The next two questions could be selected for 5 point essay questions. You will be asked to draw the operon structure. Th
questions could be selected for 5 point essay questions. You will be asked to draw the operon structure. This would include the stretch of DNA that includes genes themselves as well as upstream features that are important to each. The draw where the important proteins and enzymes would be located in relationship to the DNA strand. 1. We have discussed how the lac operon is controlled by both the CAP activator protein and the lac repressor. A) Draw and label a diagram of the lac operon (include the operator, repressor, promoter, CAP site, RNA polymerase, CAP protein, lac repressor protein, allolactose, lacZ, lacy and lacA. 1) In the presence of glucose and lactose 2) In the presence of glucose and the absence of lactose 3) In the absence of glucose and the presence of lactose 4) In the absence of glucose and the absence of lactose This means you are drawing the operon four different times, once for each of the listed conditions, indicating what might be bound or unbound to the DNA by placing the proteins and enzymes in the correct locations. AND B) State whether the lac operon will be ON or OFF under the above (4) situations: 2. (from page 333). Suppose the synthesis of an amino acid was accomplished by 4 enzymes encoded by four genes that formed an operon. Draw a model of the operon showing how it could be controlled by negative regulation of transcription initiation.
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