Mutant DNA CTTCATGACT TGTTAAGAAA GCCTACATAT ATTATTAATA TATGATTTTT CCACACCACT TGTATTATTA TATAAACCCT CTCTGTTAAC CATTTTTCTC

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Mutant Dna Cttcatgact Tgttaagaaa Gcctacatat Attattaata Tatgattttt Ccacaccact Tgtattatta Tataaaccct Ctctgttaac Catttttctc 1 (57.92 KiB) Viewed 28 times

Mutant Dna Cttcatgact Tgttaagaaa Gcctacatat Attattaata Tatgattttt Ccacaccact Tgtattatta Tataaaccct Ctctgttaac Catttttctc 2 (71.96 KiB) Viewed 28 times

Mutant Dna Cttcatgact Tgttaagaaa Gcctacatat Attattaata Tatgattttt Ccacaccact Tgtattatta Tataaaccct Ctctgttaac Catttttctc 3 (29.34 KiB) Viewed 28 times

Mutant DNA CTTCATGACT TGTTAAGAAA GCCTACATAT ATTATTAATA TATGATTTTT CCACACCACT TGTATTATTA TATAAACCCT CTCTGTTAAC CATTTTTCTC TGCTCTCTTC TCGCTGCTAC CAAGGGTTTT ACCTCACTTT CTTTCTTCAA GGACTTTATT GGTTTAAGTT GTTGGAGGCC TAATGGGTAA GGAGAAGGTT CACATTAACA TTGTTGTCAT TGGGCATGTT GACTCTGGCA AGTCAACTAC CACCGGCCAC CTCATCTACA AGCTTGGAGG TATCGACAAG CGTGTTATTG AGAGATTTGA AAAAGAAGCT GCTGAGATGA ACAAGAGGTC TTTCAAGTAT GCTTGGGTGC TGGACAAGCT CAAGGCTGAG CGTGAAAGAG GGATAACCAT TGATATTGCT CTGTGGAAGT TTGAGACCAC TAAGTACTAC TGCACAGTCA TTGATGCTCC TGGACACAGG GATTTCATCA AGAACATGAT TACTGGGACA TOCCAGGCTG ATTGTGCTGT ТСТТАТТАТТ GATTCCACCA CGGGTGGTTT TGAAGCTGGT ATTTCCAAAG ATGGACAGAC TCGTGAACAT GCTCTCCTTG CTTTCACTCT TGGTGTGAGG CAAATGATTT GCTGCTGTAA CAAGATGGAT GCCACTACAC CAAGGTACTC CAAGGCAAGA TACGATGAAA TTGTGAAGGA GGTTTCTTCC TACATGAAGA AGGTTGGATA TAACCCTGAC AAGATCCCTT TTGTTCCCAT CTCTGGTTTT GAGGGAGATA ACATGATTGA GAGGTCCACA AACCTTGACT GGTACAAGGG TCCAACCCTT CTGGAGGCCC TTGATCAGAT CAACGAGCCC AAGAGACCAT CAGACAAGCC CCTCAGATTA CCCCTTCAGG ATGTCTACAA GATTGGAGGA ATAGGAACTG TGCCTGTTGG ACGAGTTGAA ACTGGTATCA TGAAACCTGG AATGGTGGTT ACTTTTGCAC CAACTGGACT CACAACTGAA GTTAAGTCTG TGGAGATGCA CCATGAAGCA CTCACCGAGG CCCTTCCTGG TGATAATGTG GGGTTCAATG TTAAGAATGT TGCTGTTAAG GATCTCAAGC GTGGTTTCGT TGCCTCAAAC TCTAAGGATG ACCCTGCCAA GGAGGCAGCT AACTTTACCT CCCAAGTTAT CATCATGAAT CACCCTGGAC AGATTGGAAA TGGCTATGCA CCTGTTCTTG ATTGCCACAC CTCCCACATT GCTGTCAAGT TTGCCGAACT TGTGACCAAG ATTGATAGGC GTTCCGGTAA AGAGCTTGAG AAGGAGCCCA AGTTCCTGAA GAATGGAGAT GCTGGTTTTG TGAAGATGAT TCCCTCCAAG CCCATGGTGG TTGAAACCTT CTCTGAGTAT CCTCCACTTG GTCGTTTTGC TGTGAGAGAC ATGCGTCAGA CTGTGGCCGT GGGAGTCATC AAAAGCGTGG AGAAGAAGGA CCCAACTGGA GCTAAGGTCA CCAAGGCTGC ACAGAAGAAG AGTGGCAAAG GACTCGTGCA GCATGGTTTA TCAAGAGAAG TCTATCATTA CTACTACAAT AAATGGCTTC TTGTTCCTAG TACTACTATT TTTATGTTAA GGAACTTGTT TGTCGGATTT TGTGTCTGGT CTGCGCCCTC ATCTCGCGAC TTTTGTTCCC AGAACTGGGT TCTTGATCGA CGGTGGCAAT GCTATTTCTT TTTCCATTTT TATGCTTTGT TTTTATTGTA ATGTGTTTCT GTGAAAACTG TTACATTATT GTAATATAAT ATGCTGCAGA TTTGGTTTTT GAGTGTTAAT GTTAAGTTGT CAA
The DNA sequence that has been emailed to you is a mutant cDNA sequence derived from an organism that displays an altered phenotype to wild type organisms. Using appropriate bioinformatic software and databases determine the following. (1) What species is the wild type sequence derived from (give Linnaean and common species names)? 1 mark (2) What protein does for? the wild type sequence code 1 mark (3) Align the mutant and wild type cDNAs and answer the following questions. ₂. How do the mutant and wild type DNA sequences differ? b. What is the nucleotide position of the change in the mutant? 4 marks (4) Use appropriate software to deduce the protein sequence that the mutant cDNA encodes and align it with the wild type. Answer the following questions. a. What is the codon position of the change in the mutant sequence? What amino acid substitution results from the mutation? 6 marks (5) Briefly comment on the effect you think the mutation might have on the protein function using the results obtained in part 4. Limit your answer to less than 60 words. 4 marks (6) Use appropriate bioinformatic resources to find the most closely related human protein homolog of the wild type sequence. 5 marks (i) What is the percentage similarity between the wild type protein and the human protein? (ii) Use appropriate literature to find out the subchromosomal location of the human gene encoding this protein and how many exons it has. (7) Select 15 orthologs of NADH dehydrogenase subunit 3 protein sequence from an evolutionary diverse cross section of bilateria species including human and align these using Clustal. Show the Clustal alignment output obtained. Use appropriate journal literature to find features and amino acids and highlight these in the alignment. Comment on the evolutionary conservation of these in less than 150 words. 12 marks
Use Clustal X for ALL alignments and use a suitable sized font. Please ensure that you show all your working out for the above problems and cite any book or journal literature used. Do not use screenshots. Include accession numbers for all sequences used. Keep all answers as succinct as possible within the defined word limits.