What is the charge of the following peptide at pH 9.45? The pKa of the N-terminal amine is 8.0 and the pKa of the C-ter

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What is the charge of the following peptide at pH 9.45? The pKa of the N-terminal amine is 8.0 and the pKa of the C-terminal carboxylic acid is 3.5.
What is the charge of the following peptide at pH 9.45? The pKa of the N-terminal amine is 8.0 and the pKa of the C-terminal carboxylic acid is 3.5.
SANTACLAUSEISASTALKER
0
1
-1
-3
2
3
-2
best way to get an enzyme with a pI of 7.0 to stick to a cation exchange column as part of the purification protocol so that the resulting pure enzyme can be used in kinetics studies?
Which of the following conditions would be best to get an enzyme with a pI of 7.0 to stick to a cation exchange column as part of the purification protocol so that the resulting pure enzyme can be used in kinetics studies?
High salt and a pH = 7.5.
Protein has a pI of 7.0 and a pH = 7.0.
Low salt concentration and a pH = 6.5.
High salt concentration and a pH = 6.0.
Protein has a pI of 7.0 and a pH = 14.