- Tlc Identity Of An Unknown A B C D A B C D Oh Unknown Compound 10 Cm Part C Determination Of Unknown 7 Cm 5 1 (47.34 KiB) Viewed 9 times
- Tlc Identity Of An Unknown A B C D A B C D Oh Unknown Compound 10 Cm Part C Determination Of Unknown 7 Cm 5 2 (47.1 KiB) Viewed 9 times
- Tlc Identity Of An Unknown A B C D A B C D Oh Unknown Compound 10 Cm Part C Determination Of Unknown 7 Cm 5 3 (43.18 KiB) Viewed 9 times
- Tlc Identity Of An Unknown A B C D A B C D Oh Unknown Compound 10 Cm Part C Determination Of Unknown 7 Cm 5 4 (32.21 KiB) Viewed 9 times
- Tlc Identity Of An Unknown A B C D A B C D Oh Unknown Compound 10 Cm Part C Determination Of Unknown 7 Cm 5 5 (31.97 KiB) Viewed 9 times
- Tlc Identity Of An Unknown A B C D A B C D Oh Unknown Compound 10 Cm Part C Determination Of Unknown 7 Cm 5 6 (27.65 KiB) Viewed 9 times
10 cm Part C: Determination of unknown: 7 cm 5.3 cm A B 4.1 cm ● C D Solvent Front A= Tylenol B= Aspirin C= Caffeine D= Unknown
Name: Instructor: Report Sheet: Laboratory 6 Date: Section Group: Thin-Layer Chromatography PRE-LAB: The notebook pre-lab you turned in prior to conducting the experiment DATA AND OBSERVATIONS: Attach the carbon copy of your data and observations to the report sheet Part 1: Results Place your TLC plates in a plastic bag after sketching them in your notebook. Follow the instructions given for each section regarding submission of your data. Do not tape over the entire front of the TLC plate. Tape across the top and bottoms only. Part A. Anthracene vs. Fluorenone a. Tape the TLC plates of your anthracene-fluorenone separation to a sheet of paper and label them as required. Staple the paper to this report sheet. b. Report the Rf values for the following: Fluorenone 1. using 100% petroleum ether as eluant: 2. using 70/30 pet ether/diethyl ether as eluant: Anthracene 3. using 100% petroleum ether as eluant: 4. using 70/30 pet ether/diethyl ether as eluant: c. Which is more polar: anthracene or fluorenone? Why? Laboratory 6 Thin Layer Chromatography 75
d. Explain why the Rf values changed when the eluant was changed from 100% petroleum ether to a 70/30 mixture of petroleum ether/diethyl ether. Part B. Polarity Ranking Rank the compounds in order of increasing polarity (1=least polar, 3 = most polar) b. NH₂ OH Part C. Determination of an Unknown 1. Unknown ID: 2. Identify of your unknown sample: NH₂ 3-4 OH 3. In the space below, sketch the TLC plate or plates that you used to determine the identity of your known below. Be sure to label each lane on each plate and list the eluant used to develop the plate. Tape your TLC plate(s) to a piece of paper, label them appropriately, and attach the paper to this report sheet.
Part D. Short Answer Questions 1. As far as TLC is concerned, explain what is meant by the following terms: Mobile phase o Stationary phase o Origin o Solvent front 2. What is the definition of a Rf value (the retention factor)? 3. Which is the less polar of each of the following solvent pairs? Circle your answer. a. Toluene and acetone b. Dichloromethane and methanol c. Ethyl acetate and acetonitrile
Part E. Troubleshooting Questions 11 *** -808 1. In TLC plate A, describe what went wrong and troubleshoot the problem. 2. In TLC plate B, describe what went wrong and troubleshoot the problem. 3. In TLC plate C, describe what went wrong and troubleshoot the problem.