You are ready to set up your reaction. You are provided with astock of plasmid DNA that is at a concentration of 1mg/ml. You havestock enzyme buffer that is 10X (meaning it is ten-times moreconcentrated than you need it to be in the actual reaction). Youhave deionized water (dH2O) to use, as needed. As noted above, youwant to cut 2ug DNA in a reaction volume of 50ul. To make sure youget complete cutting, you’ll use an excess of enzyme so that youuse 2.5 units per microgram of DNA (2.5U/ug). The ApeKI enzyme issupplied at 5,000U/ml.
EXPLAIN THROUGHLY PLEASE!
How many ul of DNA will you add to your reaction tube?
How many ul of 10X buffer will you add to your reactiontube?
How many ul of ApeKI will you add to your reaction tube?
How many ul of dH2O will you add to your reaction tube?
At what temperature will you incubate the reaction?
You are ready to set up your reaction. You are provided with a stock of plasmid DNA that is at a concentration of 1mg/ml
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answerhappygod
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