1. You have learned how to set up a restriction endonuclease reaction to cut DNA. Suppose you are told to cut two microg
Posted: Wed Jul 06, 2022 10:34 am
1. You have learned how to set up a restriction endonucleasereaction to cut DNA. Suppose you are told to cut two micrograms(2g) of plasmid DNA with the restriction enzyme ApeKI in areaction volume of 50 microliters (50l). Go to www.neb.comto learn more about this enzyme.
A) Write below the (double-strand) recognition sequence for thisenzyme, indicating the 5’ end of each strand:
B). On the recognition sequence shown above, insert two arrowsto show where the enzyme cuts.
c) The recognition sequence includes a “W”. What does Wmean? please explain thoroughly
A) Write below the (double-strand) recognition sequence for thisenzyme, indicating the 5’ end of each strand:
B). On the recognition sequence shown above, insert two arrowsto show where the enzyme cuts.
c) The recognition sequence includes a “W”. What does Wmean? please explain thoroughly