1. You have learned how to set up a restriction endonuclease reaction to cut DNA. Suppose you are told to cut two microg

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1. You have learned how to set up a restriction endonucleasereaction to cut DNA. Suppose you are told to cut two micrograms(2g) of plasmid DNA with the restriction enzyme ApeKI in areaction volume of 50 microliters (50l). Go to www.neb.comto learn more about this enzyme.
A) Write below the (double-strand) recognition sequence for thisenzyme, indicating the 5’ end of each strand:
B). On the recognition sequence shown above, insert two arrowsto show where the enzyme cuts.
c) The recognition sequence includes a “W”. What does Wmean? please explain thoroughly