1. a) Briefly describe how recombinant DNA technology can be used to modify a wild strain (4 marks) of Escherichia coli

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1 A Briefly Describe How Recombinant Dna Technology Can Be Used To Modify A Wild Strain 4 Marks Of Escherichia Coli 1 (60.92 KiB) Viewed 13 times

1. a) Briefly describe how recombinant DNA technology can be used to modify a wild strain (4 marks) of Escherichia coli into an insulin-producing strain. b) Briefly describe three techniques that would be employed in the above procedure. (6 marks) c) List two methods for purifying the DNA from E. coli in the above procedure (la). (2 marks) (3 marks) d) Give three differences between prokaryotic and eukaryotic transcription. 2. a) Briefly discuss, with an example, how Escherichia coli protects itself against the T4 phage. (5 marks) b) Compare and contrast the similarity and difference between staggered and flush restriction digestion. (4 marks) c) Briefly discuss why ligation reactions between sticky ends are considered more effective than blunt end ligations. (4 marks) d) Give two practical advantages of dealing with blunt end ligation. (2 marks) 3. a) Briefly describe how Bacillus subtilis can be transformed to produce somatotrophin (a human growth hormone). (3 marks) b) List two criteria for assessing the success of the above procedure (3a). (2 marks)