combine with the copper in catechol oxidase and inhibit its enzymatic activity. You will determine if PTU is a competiti

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Combine With The Copper In Catechol Oxidase And Inhibit Its Enzymatic Activity You Will Determine If Ptu Is A Competiti 1 (152.08 KiB) Viewed 16 times

combine with the copper in catechol oxidase and inhibit its enzymatic activity. You will determine if PTU is a competitive or noncompetitive inhibitor of catechol oxidase. Write the question you are attempting to answer. T.S PTV a competitive in bubitor? Formulate your hypothesis. Remember, the hypothesis must be testable. PTO is a competitive inhibitor of catechol oxidase Write your prediction indicating what you expect to observe as the result of this experiment (if/then). If activity of catechol oxidase in presence of PTV is restrosed by increasing Concentration of catechol, then PTV is a competitive inhibitor Procedure Set up your experiment using the following table. Label 3 tubes. Add the contents to each tube following the presented order of the table from left to right. (Note: 1 mi is equivalent to 20 drops; 10 drops are equivalent to 0.5 ml). (Your instructor may want you to wait for 1 minute after PTU before adding the Catechol) Tube 1 2 3 Distilled water 5 ml 5 ml 5 ml Potato extract (Enzyme Sources) 10 drops 10 drops 10 drops PTUS (Inhibitor) 10 drops 10 drops Distilled water 10 drops Catechols (Substrate) 38 10 drops 20 drops 10 drops 20 drops ($) Catechol and PTU are toxic! Avoid skin contact with solutions. Do not pipette any solution by mouth. Wash your hands thoroughly after the experiment! 1. Which tube is the negative control for inhibition? Tube 1 and 2 is the negative control for inhibition. 2. Why is it included? Because hibe 1 and 2 has the solution that one not change to brown color 3. What are the necessary components for the inhibition to take place? -potato extract and catechol 4.Explain the importance of adding PTU to the potato extract before catechol? 5. What is the difference between the contents of tubes 1 and 2 that is necessary for you to determine if PTU is a competitive or non-competitive inhibitor? Use the space to present your description of the qualitative data in Table form showing only the independent and dependent variables. (Tube numbers are not independent variables, refer to them by the important content(s) responsible for the result. Water is present in all and is not an independent variable)