5'...AGATTCGAATCT...3. Q18. In E. coli, the leuB' gene is needed to make leucine and leuB-mutants are auxotrophic for le

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5 Agattcgaatct 3 Q18 In E Coli The Leub Gene Is Needed To Make Leucine And Leub Mutants Are Auxotrophic For Le 1 (23.95 KiB) Viewed 12 times

5'...AGATTCGAATCT...3. Q18. In E. coli, the leuB' gene is needed to make leucine and leuB-mutants are auxotrophic for leucine. Part of the wild- type leuB open reading frame has the sequence: 5...AGATTCGGATCT...3. At the same position, the F-leuB-mutant strain has the sequence: Three different F' strains, F' leuB-1 through F' leuB-3, each carrying a different mutant leuB allele on the F factor, were independently crossed to the FleuB strain by conjugation. After conjugation was complete the cells were plated onto medium lacking leucine to select for leuB recombinants. The cross using which F' plasmid produced leuB* recombinants? Borders A. F'leuB-1: 5'... AGATTCGATCT...3' 5'...AGATACGGATCT...3 B. F'leuB-2: C. F'leuB-3: 5'...AGATTCGAATCT...3'