- A Plasmid Size 10592 Bp Cut By Two Restriction Enzymes Ecor1 And Hind Iii Draw The Pattern Of Bands After Single And Dou 1 (311.71 KiB) Viewed 14 times
- A Plasmid Size 10592 Bp Cut By Two Restriction Enzymes Ecor1 And Hind Iii Draw The Pattern Of Bands After Single And Dou 2 (286.7 KiB) Viewed 14 times
A plasmid size 10592 bp cut by two restriction enzymes EcoR1 and Hind III Draw the pattern of bands after single and double digestion with both enzymes on gel. And also add restriction sites on plasmid map. Restriction site Site 1 Site 2 Site 3 Site 4 Site 5 Site 6 EcoR1 874 968 2405 3552 7107 8305 Hind III 650 905 1423 3201 Mention restriction sites in this Circle 10592bp plasmid 1
10000 9000 8000 ● 7000 6000 5000 4000 3000 2000 1000 500 400 300 200 Instructions: 1kb ladder EcoR1 Hind III EcoR1+Hind III Locate the restriction sites on plasmid map. Draw separate map for each restriction enzyme. (3 Marks) Calculate the product size with the help of restriction sites. (Show your Calculations) (4.5 Marks) Draw the restricted product/bands on the gel image and label with size. (1.5 + 1 Marks for proper labelling)