Background C Type Lysozymes Catalyze The Hydrolysis Of The 1 4 Beta Linkages Between N Acetyl D Glucosamine And N Acet 1 (53.96 KiB) Viewed 13 times
After purification, you and your partner had stored the proteins away in the freezer for
use the following week. However, had not used permanent markers or cryo-labels and the
writing had smudged. How would you identify each of your samples?
Background C Type Lysozymes Catalyze The Hydrolysis Of The 1 4 Beta Linkages Between N Acetyl D Glucosamine And N Acet 2 (47.56 KiB) Viewed 13 times
Background C-type lysozymes catalyze the hydrolysis of the 1, 4-beta-linkages between N-acetyl- D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls and between N-acetyl-D-glucosamine residues in chitodextrins. C-type lysozymes are closely related to a-lactalbumin in sequence and structure making them part of the same family. The sequence comparison of a-lactalbumin shows a strong similarity to that of lysozymes, specifically the Ca-binding c- lysozyme. However, the c-Lysozyme in this experiment does not bind Ca suggesting that the expected evolutionary history is that gene duplication of the c- lysozyme was followed by mutation. A search for c type lysozyme proteins in mammalian systems revealed Sperm lysozyme like protein (SLLPI) in mouse and human (HSLLP1). These proteins are implicated in the fusion of the sperm and ova. However, SLLP1 proteins do not exhibit the catalytic activities exhibited by the c- type lysozymes. The functional difference between the SLLP1 proteins and HEW lysozyme C are attributed to the mutations of catalytic residue glutamine at position 35 to threonine (Q35T) and residue aspartic acid at position 52 to asparagine (D52N). The sequences of each of these proteins are given below. Sequence of human a-lactalbumin [P00709] MRFFVPLFLV GILFPAILAK QFTKCELSOL LKDIDGYGGI ALPELICTMF HTSGYDTQAI 70 ChemL-301 Case Study Summer 2022 Case Study 130 70 80 130 VENNESTEYG LFQISNKLMC KSSQVPOSRN ICDISCOKFL DODITODIMC AKKILDINGI 140 80 90 DYWLAHKALC TEKLEQWLCE KL Sequence of Lysozyme C [P61626] MKALIVIGLV LLSVTVOGKV FERCELARTL KRLGMDGYRG ISLANWMCLA KWESGYNTRA 140 100 90 ChemL-301 Fall 2011 Case study 110 100 PQGIRAWVAN RNRCONRDVR QYVOGCGV Human Sperm lysozyme like protein [Q8IXA5-2] 120 TNYNAGORST DYGIFQINSR YWCNDGKTPG AVNACHLSCS ALLQONIADA VACAKRVVRD 110 120
MLLALVCLLS CLLPSSEAKL YGRCELARVL HDFGLDGYRG YSLADWVCLA YFTSGENAAA 70 80 130 140 90 GLGYWEAWRH HCQGKDLTEW VDGCDF 100 LDYEADGSTN NGIFQINSRR WCSNLTPNVP NVCRMYCSDL LNPNLKDTVI CAMKITOEPQ 110 120 Discuss the objective of your case and experiments you decide to use to solve the same. Write a plan to perform the task and in necessary list an alternative plan too. Objectives: 1. After purification, you and your partner had stored the proteins away in the freezer for use the following week. However, had not used permanent markers or cryo-labels and the writing had smudged. How would you identify each of your samples?
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