or converted to a PDF. Purpose: This assignment will prepare you for doing structural stains in lab exercises 3-8 (Capsu

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Or Converted To A Pdf Purpose This Assignment Will Prepare You For Doing Structural Stains In Lab Exercises 3 8 Capsu 1 (63.66 KiB) Viewed 11 times

Or Converted To A Pdf Purpose This Assignment Will Prepare You For Doing Structural Stains In Lab Exercises 3 8 Capsu 2 (82.46 KiB) Viewed 11 times

or converted to a PDF. Purpose: This assignment will prepare you for doing structural stains in lab exercises 3-8 (Capsule Stain) and 3-9 (Spore Stain). You will interpret what the results of the staining procedures mean and you will consider what purpose these structures serve for bacterial cells. Task: ● . Watch the following introductory videos: o Capsule Stain o Endospore Stain o Note that we will not stain our samples in the sink as shown in the videos. Due to new environmental safety regulations, we will stain our samples over a cake pan and dispose of the stain waste in a container at the end of lab. Please follow all in-lab instructions. . Prelab 4 (3 pts): Structural stains (3-8 and 3-9) Answer the following questions in your own words before coming to lab. • Upload this Word or PDF document containing your bolded answers directly into Canvas. . Criteria for Success: Be prepared for lab: read the pages of the lab manual indicated above and carefully watch the assigned video(s). • Make yourself a "sticky note" with a brief version of following protocols: o The capsule stain steps & timing. o The endospore stain steps & timing. Always read all of the instructions. Answer all parts of all questions. Meet your deadline. . Read the following pages in your lab manual: o 201-202 (Capsule Stain, 3-8). . o 205-208 (Endospore Stain, 3-9). o Chapter 3 of the textbook may also be helpful.
1. The procedure for staining for capsules is different than the procedure used for most other stains. a. When we do a capsule stain with Congo red, we start with a drop of the slide, add to it, and then use and bacteria down the slide. b. We will suons: (always, sometimes, or never?) heat fix the Congo red slide because the bacteria (do what?), which causes them to look like they have a (what cellular structure?), even when they do not. This will lead to false positives and will be misleading. 2. Consider the procedures and the final results of both the capsule stain and the endospore stain. a. Reflect on the relative ease of capsule staining bacterial cells with Maneval's stain (in the fume hood). For the Maneval's stain, we, (do or do not?) use a steam bath. We stain with Maneval's stain for (how long?) and we do this part of the capsule stain in the fume hood because Maneval's stain contains phenol that, (is or is not?) good for us to inhale. b. Maneval's stain I predict that capsules surrounding the bacteria from antibiotics. on the edge of to spread the Congo red d. I think that endospores, (does or does not?) stain the bacteria inside of a capsule. Therefore, (do or do not?) protect the bacteria c. Reflect on the relative ease of staining an endospore. For the malachite green stain, we (do or do not?) use a steam bath for manual says. Stain with malachite green for as long as the video indicates). (present your evidence). (how long? Note that this differs from what your lab (do or do not?) protect bacteria from antibiotics, because