- 2 A 3 A 4 B Explain Why 5 Did The Color Change In The Beaker The Dialysis Bag Or Both In Procedure 6 1 A For 1 (39.93 KiB) Viewed 15 times
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Figure 6.2: Prep Table 6.2: Shows color change of solutions found either in the dialysis bag or in the beaker. Solutions Initial Color Final Color Water + lodine yellow yellow Starch solution Clear Purple Beaker Dialysis Bag Conclusions Questions 1. Did the color change in the beaker or the dialysis bag? Explain why this happened. 2. How is the dialysis tubing different than a cell membrane? Osmosis Osmosis is the net movement of water across a selectively permeable membrane from an area of low solute concentration to an area of high solute concentration. Another way of looking at this is that water is moving through a selectively permeable membrane from an area of high water concentration to an area of low water concentration, so osmosis is effectively the diffusion of water. The rate at which water or any other molecule moves depends on the concentration gradient between those two areas. The greater the concentration gradient, the faster the diffusion rate. For
water Bag A Bag B Bag C Bag D 2556 sucrose Bag A o m 1 nucóse Dialysis Bag Contents 10 mL of 1% sucrose solution 10 mL of 1% sucrose solution 10 mL of 10% sucrose solution 10 mL of 25% sucrose solution Table 6.3: Contains total weights of dialysis bags collected at different time points. 15 sucrose Beaker Beg B 10ml 16 subse A 25% Sucrose Solution Figure 6.3: Experimental setup up for Procedure 6.2. Beg C hom 10% supose Initial Weight (g) 549 to mu 25% supose 15 min 30 min 45 min Weight (g) 60 min Weight (g) Weight Weight (g) (g) 15.29 14.19 1.59 12.39| 11.49 bluedoor, LLC B 1% Sucrose 10.5916.89 11.89 11.09 10.29 Solution B 1% Sucrose 10.79 22.3g|11.99|11.69|11.59g. Solution B 196 Sucrose 18.79 18.19 14.6g 14.29 21.34 Solution Questions 1. In which dialysis bags did water move across the membrane? How were you able to determine this? Laboratory & Diffusion and Osmosis 71
8. 9. 10. 11. 12. Allow the potato cylinders to sit for 60 minutes at room temperature, making sure to swirl the beakers every 20 minutes. After 60 minutes, remove the potato cylinder from the 0% NaCl beaker and gently dry it using a piece of paper towel. Weigh the potato cylinder to the nearest 0.1 grams and record the final weight in Table 6.4. Repeat steps 9-10 for the remaining two beakers. Calculate the percent change in weight by subtracting the initial weight from the final weight and then dividing by the initial weight. This number is then multiplied by 100. Reference the equation below. Percent change in weight = Final weight-Initial weight x 100 Initial weight Table 6.4: Effect of salt concentration on potato weight. Concentration of Salt Solution 0% 0.9% 5% Initial Weight of Potato Cylinder (g) 13.29 16.49 14.19 Final Weight of Potato Cylinders (g) 13.29 16-2д 13.79 Percent Change in Weight 6.2 g 9.19 6.69 Procedure 6.5 Graph the Osmosis Data 1. Using the graph paper on the next page, construct a graph displaying the relationship between concentration of salt solution (%) and percent change in weight. 2. Remember the independent variable is always graphed on the x-axis and dependent varia on the y-axis. Include a description, correctly labeled axes, and values along each axis.