You are attempting to grow a culture of Escherichia coli to use in an experiment, and you need the culture to reach 106

[answerhappygod]

You Are Attempting To Grow A Culture Of Escherichia Coli To Use In An Experiment And You Need The Culture To Reach 106 1 (347.96 KiB) Viewed 11 times

You are attempting to grow a culture of Escherichia coli to use in an experiment, and you need the culture to reach 106 cells/mL before you can use them. In your lab, E. coli doubles every 25 minutes. How long will it take a culture you inoculate at 10² cells/ml to grow up to your desired density? About 3 hours About an hour and a half About five and a half hours About 7 hours 问题 20 Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD reading for them. You do this favor for them, and helpfully do the conversion into cells/mL, finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus doubles every 5 hours. They beg you to make a new culture using these cells and set it up to reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for three hours upon dilution into fresh medium, what cell density should you dilute the culture to? About 1.3 x 107 cells/ml About 1.3 x 104 cells/ml About 1.3 x 105 cells/ml 15 About 1.3 x 106 cells/ml