1. (6 points; 2 points each) Assume a digest was conducted on pUC18 with EcoR1 and Hind!!!. Note: Use addgene.org to loo

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1 6 Points 2 Points Each Assume A Digest Was Conducted On Puc18 With Ecor1 And Hind Note Use Addgene Org To Loo 1 (58.59 KiB) Viewed 10 times

1. (6 points; 2 points each) Assume a digest was conducted on pUC18 with EcoR1 and Hind!!!. Note: Use addgene.org to look up this plasmid's restriction map to answer the questions below. Include this as a reference in the Reference section of this worksheet. a. Draw a plasmid restriction map for pUC18 with the EcoR1 and Hind!!! restriction sites in the appropriate places. Include the length (in bp or kb) of the entire plasmid. pUC18 b. What is the DNA sequence for each restriction site? What type of end would each form? Include a reference in the Reference section of this worksheet. EcoR1: Hindill: wun