You are performing a bacterial cloning experiment using theβ-Galactosidase/X-Gal system discussed in class. After doing theexperiment to ligate your DNA insert into the plasmid vector, youperform a heat-shock transformation of the E.coli bacteria, plate your cells into a media withantibiotic and X-Gal, and incubate the plates overnight. The nextmorning, you take your plates from the incubator and notice abacterial mat, i.e. no individual colonies. Is there somethingwrong? If so, what went wrong?
You are performing a bacterial cloning experiment using theβ-Galactosidase/X-Gal system discussed in class. After doing theexperiment to ligate your DNA insert into the plasmid vector, youperform a heat-shock transformation of the E.coli bacteria, plate your cells into a media withantibiotic and X-Gal, and incubate the plates overnight. You decideto repeat the experiment from the previous question. The morningafter repeating the experiment, you see a plate with lots of bluecolonies, but no white colonies. Is there something wrong? If so,what went wrong?
Read the following sequencing gel radiography to the right, andwrite the first 10 bases, from the 5'-end.
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CGAT 1.00 DIA 71 ||||
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