1a.. Explain the purpose behind the steps taken during Western blotting. - blocking the membrane - incubation with prima
Posted: Wed Mar 09, 2022 9:32 am
1a.. Explain the purpose behind the steps taken during Western
blotting.
- blocking the membrane
- incubation with primary antibody
- incubation with AP-conjugated secondary antibody
- NBT/BCIP
- washing with TBST
1b. What effect does cisplatin-treatment have on the expression
of p53 in LLC-PK1 cells?
blotting.
- blocking the membrane
- incubation with primary antibody
- incubation with AP-conjugated secondary antibody
- NBT/BCIP
- washing with TBST
1b. What effect does cisplatin-treatment have on the expression
of p53 in LLC-PK1 cells?