1a.. Explain the purpose behind the steps taken during Western blotting. - blocking the membrane - incubation with prima

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answerhappygod
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1a.. Explain the purpose behind the steps taken during Western blotting. - blocking the membrane - incubation with prima

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1a.. Explain the purpose behind the steps taken during Western
blotting.
- blocking the membrane
- incubation with primary antibody
- incubation with AP-conjugated secondary antibody
- NBT/BCIP
- washing with TBST
1b. What effect does cisplatin-treatment have on the expression
of p53 in LLC-PK1 cells?
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