1a. RIPA (Radio-immunoprecipitation assay) lysis buffer consists of Tris (pH 7.4), sodium chloride, EDTA, sodium fluorid
Posted: Wed Mar 09, 2022 9:32 am
1a. RIPA (Radio-immunoprecipitation assay) lysis buffer consists
of Tris (pH 7.4), sodium chloride, EDTA, sodium fluoride, sodium
pyrophosphate, sodium orthovanadate, Triton-X, sodium lauryl
sulphate (SDS), phenylmethylsulfonylfluoride (PMSF), aprotinin,
pepstatin, and leupeptin. State the function of each of these
chemicals in the lysis buffer.
1b. SDS-PAGE gels contain Polyacrylamide
(acrylamide/bisacrylamide) stock, Tris, SDS, ammonium persulphate
and TEMED. Explain how these chemicals contribute to the
polymerization/ running of the gel in western blot?
1c. Why is it necessary to transfer the proteins to
nitrocellulose membrane instead of probing the gel directly?
of Tris (pH 7.4), sodium chloride, EDTA, sodium fluoride, sodium
pyrophosphate, sodium orthovanadate, Triton-X, sodium lauryl
sulphate (SDS), phenylmethylsulfonylfluoride (PMSF), aprotinin,
pepstatin, and leupeptin. State the function of each of these
chemicals in the lysis buffer.
1b. SDS-PAGE gels contain Polyacrylamide
(acrylamide/bisacrylamide) stock, Tris, SDS, ammonium persulphate
and TEMED. Explain how these chemicals contribute to the
polymerization/ running of the gel in western blot?
1c. Why is it necessary to transfer the proteins to
nitrocellulose membrane instead of probing the gel directly?