3a-i: In connection with method development for reverse phase liquid chromatography (HPLC) analyzes a sample containing

Business, Finance, Economics, Accounting, Operations Management, Computer Science, Electrical Engineering, Mechanical Engineering, Civil Engineering, Chemical Engineering, Algebra, Precalculus, Statistics and Probabilty, Advanced Math, Physics, Chemistry, Biology, Nursing, Psychology, Certifications, Tests, Prep, and more.
Post Reply
answerhappygod
Site Admin
Posts: 899603
Joined: Mon Aug 02, 2021 8:13 am

3a-i: In connection with method development for reverse phase liquid chromatography (HPLC) analyzes a sample containing

Post by answerhappygod »

3a I In Connection With Method Development For Reverse Phase Liquid Chromatography Hplc Analyzes A Sample Containing 1
3a I In Connection With Method Development For Reverse Phase Liquid Chromatography Hplc Analyzes A Sample Containing 1 (43.4 KiB) Viewed 51 times
3a-i: In connection with method development for reverse phase liquid chromatography (HPLC) analyzes a sample containing 10 compounds using one linear test gradient. The gradient time is 30.00 min. The first connection gets then a retention time of 6.85 min, the last compound elutes after 28.42 min from the column. Should isocratic elution or gradient elution be used in the future the method development work? The answer must be substantiated. 3a-ii: What effect will it have on the solubility in gas chromatography by switching from an open capillary column (WCOT) with a large inner diameter and thick stationary phase film to an open capillary column with small inner diameter and thin stationary phase film?
Join a community of subject matter experts. Register for FREE to view solutions, replies, and use search function. Request answer by replying!
Top
Post Reply

Return to “Archived Questions & Answers”