What is the correct order to prepare pure recombinant proteins when using E coli expression system? 1. Select appropriat

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What is the correct order to prepare pure recombinant proteins
when using E coli expression system?
1. Select appropriate column chromatography to purify the
recombinant proteins
2. Make a DNA plasmid containing gene that codes target
protein
3. Transform the DNA plasmids into E. coli cells
3. Grow E. coli cells in appropriate media for overexpressing
recombinant proteins by IPTG and harvest cells
4. Break open the cells to release the recombinant proteins into
appropriate buffer and spin down to get supernatant part