A bottle contains a mix of three amino acids in citrate buffer (pH 3.5). The amino acids in solution are Lysine, Valine,

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A bottle contains a mix of three amino acids in citrate buffer
(pH 3.5). The amino acids in solution are Lysine, Valine, and
Aspartate.


If a cation exchange column was set up and equilibrated with
citrate buffer (pH 3.5) state which amino acid(s) would bind to the
column and which amino acid(s), if any, would flow through the
column. Explain your reasoning. Word limit: 150 words.


Note that the Isoelectric point (pI) at 25 o C
of the amino acids are:
Lysine (pI 9.47)
Valine (pI 6.02)
Aspartate (pI 2.98)