Neural activation of skeletal muscle involves a sequence of steps. Understanding the steps in this sequence helps you to

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Neural activation of skeletal muscle involves a sequence of
steps. Understanding the steps in this sequence helps you to
determine the locus of disturbances that affect muscle contraction.
You are given an isolated muscle with its motor nerve intact. This
preparation is arranged so that you can make electrophysiological
measurements from the nerve and muscle as well as mechanical
measurements of muscle contraction. You observe that if caffeine is
added to the fluid bathing the muscle preparation, the peak tension
developed in the muscle twitch produced by stimulating the motor
nerve is increased.
4. If you were to induce muscle contractions at a level
that mimics high-intensity exercise (90% maximum), what fuel(s)
would you need to provide the muscle? How might that change if you
were to mimic 20% exercise intensity?
5. If you accidentally add too much caffeine to the
preparation, the muscle stays contracted. Explain what is happening
and what you might expect to see after some time
6. A motor unit includes a neuron and all of the
muscle fibers it innervates. Explain how the innervations and
measurements of muscle tension might differ if the preparation was
an eye muscle as compared to the quadriceps. (Hint: consider motor
units)