1. The is term used for the accumulation of bacteria that is collected from the culture tubes and then moved into a new

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1 The Is Term Used For The Accumulation Of Bacteria That Is Collected From The Culture Tubes And Then Moved Into A New 1 (63.72 KiB) Viewed 229 times

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1. The is term used for the accumulation of bacteria that is collected from the culture tubes and then moved into a new medium in order to produce a culture. (1 point) 2. Describe the method used to inoculate each of the following types of media: (3 points) a. Broth b. Slant c. Deep 3. What is the purpose of the agar added to certain media? (1 point) 4. To decrease the likelihood of contamination, how should you hold your agar plates when working with them? (1 point) 5. In order for the autoclave to achieve maximum sterility, it is important to achieve a temperature of and a pressure of for about 30 minutes. (2 points) Lab #3 6. Basic dyes are used to perform simple and differential staining techniques because they employ a -charged chromogen that is electrostatically attracted to the charges of the bacterial surface. (2 points) 7. Provide two (2) examples of basic dyes commonly used in the Microbiology lab. (2 points) 8. A stain is typically used to identify the true shapes of cells because the nigrosin will not bind to the cell and will color the background. (1 point) must first be 9. Before viewing your specimen with the 100X lens, added to the slide to prevent light from refracting and not entering the lens. (1 point) 10. Prior to heat-fixing your specimen to the slide, it is crucial that you first allow plenty of time for the smear to completely. dry. (1 point) 11. Provide a simple explanation of how the process of heat-fixing your slide allows your specimen to adhere to the slide better than if this step is omitted. (1 point)
12. When placing your scopes away at the end of the lab session, you should ensure that the objective lens is directly over the stage and that the has been adjusted to its lowest setting to prevent damage to the bulb when it is turned on again. (2 points) Lab #4 13. What is the function of a mordant when performing various differential stain techniques? What is the mordant in the Gram staining technique? (2 points) 14. When the Gram staining technique is performed correctly, bacteria that stain purple with the crystal violet are classified as Gram- bacteria. (1 point) 15. After the addition of the 95% Alcohol, all of the bacteria that are considered to be Gram- will be colorless, until the counterstain is applied. (1 point) 16. The differential staining technique is useful in identifying bacteria possessing mycolic acid (wax) in their cells walls. A clinically important genus of bacteria that has mycolic acid in its cell walls is (2 points) 17. Clostridium and Bacillus are both clinically important genera of bacteria that produce tough structures used to protect their DNA during times of environmental stress. (1 point) 18. A media contains inhibitory agents that prevent or significantly reduce the growth of certain kinds of bacteria. (1 point)