- B Western Blot Method For B After Transfer Of Proteins To Nitrocellulose Laboratory Staff Will Incubate The Membrane 1 (300.71 KiB) Viewed 64 times
After transfer of proteins to nitrocellulose, laboratory staff
will incubate the membrane with one of the following antibodies: 1.
Rabbit monoclonal anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
2. Rabbit monoclonal anti-p44/42 MAPK (Erk1/2) The antibodies will
be diluted in a mixture of isotonic salt solution of 5% (w/v) of
skim milk powder and incubated overnight at 40C
--- OH O RAS phospho-ERK Total ERK MW A549 BEAS2B A549 BEAS2B A549 BEAS2B MW A549 BEAS2B A549 BEAS2B