Please Interpret Each Of The Genetic Results Method For A Pcr Reactions To Be Prepared 1 2 3 4 5 6 Annealing Temperatur 1 (335.74 KiB) Viewed 155 times
Method for A.
PCR reactions to be prepared
1
2
3
4
5
6
Annealing temperature
60oC
X 5 cycles
63oC
X 5 cycles
66oC
X 5 cycles
56oC
X 30 cycles
59oC
X 30 cycles
62oC
X 30 cycles
Genomic DNA (ng)
25
25
25
25
25
25
Primers
(final concentration for each primer in µM)
0.2
0.4
0.2
0.4
0.2
0.4
500- 400- bp 1 2 3 4 5 6 7 8 9 10 11 12 1
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