Question 5. 3 marks This question is about real-time PCR This graph represents the results from a Real-time PCR of brain

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Question 5 3 Marks This Question Is About Real Time Pcr This Graph Represents The Results From A Real Time Pcr Of Brain 1 (78.77 KiB) Viewed 170 times

Question 5. 3 marks This question is about real-time PCR This graph represents the results from a Real-time PCR of brain sample form a patient who died of Herpes simplex virus encephalitis, using primers against the HSV DNA polymerase gene. The Green and Blue lines are the controls, one with 10 vira genomes, the other with 100,000 vira genomes. 35 30 25 20 15 10 0.0 10 50 Cycles log Copy Number What is the purpose of the experiment? [1 mark] The purpose of the experiment is to... What does the Green curve correspond to? 10 or 100,00 viral genomes? Why? [0.5 mark] The green curve corresponds to... ...because... What does the Blue curve correspond to? 10 or 100,000 viral genomes? [0.5 mark] The blue curve corresponds to... ...because... 1