Structure of Fast Green FCF In this experiment you are given four different concentrations of Fast Green FCF. The bottle

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Structure Of Fast Green Fcf In This Experiment You Are Given Four Different Concentrations Of Fast Green Fcf The Bottle 1 (58.08 KiB) Viewed 205 times

Structure of Fast Green FCF In this experiment you are given four different concentrations of Fast Green FCF. The bottle marked 0.125 mM represents a known concentration of a protein stained with a green dye. The other three are unknown protein solutions dye. Your is to the concentrations the unknown solutions using a standard curve of known concentrations that you generate. You will use 0.1M Tris to dilute the protein stained with green dye. 1. Make the following solution: 200 mL of 0.1 M Tris, pH = 8.5 (Tris base, MW= 121.14). Don't forget to properly label your solution and store any remaining solution for later use. 2. Prepare your standard curve of known concentrations by diluting from your 0.125 mM stock. You will want to prepare at least 2 mL of each standard to ensure the light passes through your sample (rather than through the air above your sample) while in the spectrophotometer. Obtain 7 cuvettes and label each one with tape. Prepare the following dilutions from your 0.125 mM stocks into your labeled cuvettes: Cuvette # 0.125mM stock (mL) Concentration 0.1M Tris (ml) 1 0.625 µM 1.99 0.01 1.98 0.02 1.25 μM 2 1.96 0.04 2.5 μM 3 1.92 0.08 5.0 μM 4 0.12 1.88 5 7.5 µM 0.16 1.84 6 10 μM 1.80 0.20 7 12.5 μM 3. Cover each cuvette with a piece of parafilm and mix by inversion.