In this exercise you must create an experimental design to investigate how the mutation in S. cerevisiae will affect the
Posted: Thu Jun 09, 2022 11:53 am
In this exercise you must create an experimental design to
investigate how the mutation in S. cerevisiae will affect the
cell’s function
Protein: KGD1 / YIL125W (catalyzes a key step in the
tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of
alpha-ketoglutarate to form
succinyl-CoA 2)
1. Identify a method, explain why it is appropriate, and
accurately describe advantages and limitations.
2. Clearly outline appropriate experimental controls, possible
areas of error and describe expected outcomes.
investigate how the mutation in S. cerevisiae will affect the
cell’s function
Protein: KGD1 / YIL125W (catalyzes a key step in the
tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of
alpha-ketoglutarate to form
succinyl-CoA 2)
1. Identify a method, explain why it is appropriate, and
accurately describe advantages and limitations.
2. Clearly outline appropriate experimental controls, possible
areas of error and describe expected outcomes.