In this exercise you must create an experimental design to investigate how the mutation in S. cerevisiae will affect the

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In this exercise you must create an experimental design to
investigate how the mutation in S. cerevisiae will affect the
cell’s function
Protein: KGD1 / YIL125W (catalyzes a key step in the
tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of
alpha-ketoglutarate to form
succinyl-CoA 2)
1. Identify a method, explain why it is appropriate, and
accurately describe advantages and limitations.
2. Clearly outline appropriate experimental controls, possible
areas of error and describe expected outcomes.